FIG. 4.

3-D image reconstruction of rcISVPs obtained from cryo-TEM images (E and F; 33-Å resolution) compared with previously reported reconstructions of T1L virions (A and B; 28-Å resolution) and ISVPs (C and D; 29-Å resolution) (21). (A, C, and E) Surface-shaded views down a twofold axis of symmetry for each particle. (B, D, and F) Same views as in panels A, C, and E except that the reconstructions were radially cropped to remove all densities below 41 nm, thereby isolating features attributable to ς3 in the display. Bar, 30 nm.