FIG. 8.

Capacity of rcISVPs to be activated to mediate hemolysis or transcription. Each bar represents the mean ± standard deviation from three trials. (A) A 3% solution of calf erythrocytes was incubated with 5 × 10¹⁰ T1L virions (vir), rcISVPs (rcI), or ISVPs generated by CHT digestion of each (vir + CHT or rcI + CHT, respectively) at 37°C for 30 min in the presence of 200 mM CsCl as an accelerant (8, 15). The extent of hemolysis was expressed as a percentage of that obtained by hypotonic lysis. (B) T1L virions, rcISVPs, or their respective ISVPs (5 × 10¹⁰ each) were incubated with a transcription mixture including 2 mM ribonucleoside triphosphate, 1.13 μCi of [α-³²P]GTP, and 200 mM CsCl. The incorporation of ³²P into viral transcripts was measured after precipitation with trichloroacetic acid (36).