FIG. 3.

Detection of EBNA-1 in B-cell clones carrying recombinant EBV. (A and B) Immunoblot analysis of protein extracted from 100,000 cells of different B-cell clones that were immortalized with EBV that had recombined with the indicated plasmids. The numbers above the lanes of the gels designate specific clones, most of which were also analyzed for viral DNA in Fig. 2 and 4. EBNA-1 of the B95-8 size and of the dl7 size are indicated by EBNA1 and dl7, respectively, to the left. Louckes is an EBV-negative Burkitt’s lymphoma cell line. EBV of Raji has fewer IR3 repeats than B95-8 EBV, so its EBNA-1 is correspondingly smaller. DF1 is a human B-cell clone immortalized with B95-8 EBV.