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. 2023 Jul 24;10:1223436. doi: 10.3389/fvets.2023.1223436

Table 1.

Enrichment of phage clone output in three screening rounds.

Ph.D. 12 mer (pfu/mL) Ph.D. c7c (pfu/mL)
Coating antibody Eluted phage titer Increase in phage titer (fold) Amplified phage titer Eluted phage titer Increase in phage titer (fold) Amplified phage titer
1st Pannig (10 μg/well) 2.0 × 105 1.0 3.0 × 1013 1.5 × 105 1.0 4.8×1013
2nd Pannig (5 μg/well) 2.8 × 106 14.0 3.0 × 1012 3.3 × 105 2.2 5.0 × 1012
3rd Panning (2.5 μg/well) 6.9 × 106 34.5 N.A. 2.2 × 107 146.7 N.A.

pfu plaque forming units; N.A. not amplified.