Table 3.
Peptide deduced aminoacid sequences on phage clones selected cyclic 7 mer library after three screening rounds.
| Cyclic 7-mer Phage selected clones | *AA sequence | Identified protein, sequence ID, residues length and number of matches | Position/protein coverage |
|---|---|---|---|
| 1,5 | CNTGSPYEC | Microneme protein 4 [E. tenella] Sequence ID: CAC34726.2 Length: 2340 Matches: 10 | Position 297, 991/5/8 (63%) Position 769, 810, 938, 124/6/9 (67%) |
| 6 | CMSTGLSSC | Thrombospondin type 1 domain-containing protein, putative [E. tenella] Sequence ID: XP_013235772.1 Length: 3774. Matches: 5 | Position 3,575/7/9 (78%) |
| 7,15,20 | CSISSLTHC | Hypothetical protein, conserved [E. tenella] Sequence ID: XP_013233649.1 Length: 961. Matches: 3 | Position 412/7/8 (88%) |
| 8 | CRSANIYTC | Hypothetical protein, conserved [E. tenella] Sequence ID: XP_013232311.1 Length: 1348. Matches: 1 | Position 1,270/5/7 (71%) |
| 9,16 | CHPVSGQKC | Hypothetical protein ETH_00011430 | Position 208;/6/7(86%) |
| 10,18 | CLKFWKPNC | Hypothetical protein, conserved [E. tenella] Sequence ID: XP_013228358.1 Length: 323 Matches: 1 | Position 185/5/7(71%) |
| 7′ | CLKLGEKWC | Hypothetical protein ETH_00039830 [E. tenella] Sequence ID: XP_013231892.1 Length: 271 Matches: 1 | Position 128/6/7 (86%) |
| 10′ | CAKLCLNNC | Asparaginyl-tRNA synthetase, putative [E. tenella] Sequence ID: XP_013227989.1 Length: 516 Matches: 1 | Position 319/6/9 (67%) |
| 11 | CHQTKTKFC | Hypothetical protein ETH_00037435 [E. tenella] Sequence ID: XP_013229950.1 Length: 251 Matches: 1 | Position 137/6/6 (100%) |
| 13 | CHNETQKMC | Dynein heavy chain 3, axonemal, related [E. tenella] Sequence ID: XP_013232337.1 Length: 1111 Matches: 1 | Position 462/6/9 (67%) |
| 17 | CVGISALLC | Adenosine transporter, putative [E. tenella] Sequence ID: XP_013231508.1 Length: 443 Matches: 1 | Position 197/7/9 (78%) |
| 1′,3′ | CPTNQHHLC | Regulator of chromosome condensation RCC1 (Precursor), related [E. tenella] Sequence ID: XP_013236104.1 Length: 812 Matches: 1 | Position 26/6/8 (75%) |
| 2′ | CMNNFNITC | Hypothetical protein, conserved [E. tenella] Sequence ID: XP_013233414.1 Length: 1063 Matches: 1 | Position 1/6/8 (75%) |
*
Consensus amino acid residues are highlighted in bold letters. Position and protein coverage for AA sequence of every selected phage clone on NCBI standard protein BLAST protein►protein sequence of the Eimeria tenella taxid 5,802.