Skip to main content
. 2023 Aug 4;11:e15798. doi: 10.7717/peerj.15798

Figure 3. Exos-shVDR promoted macrophage M1 polarization.

Figure 3

(A) The expression levels of macrophage M1 polarization marker (CD86) and M2 polarization marker (CD206) in macrophages were measured by flow cytometry. N = 3. (B) The mRNA expression levels of macrophage M1 polarization markers (IL-1β, TNFα, IL-6) and M2 polarization marker (IL-10) in macrophages were detected by RT-qPCR. N = 3. (C) ELISA was applied to detect the content of macrophage M1 polarization markers (IL-1β, αTNFα, IL-6) and M2 polarization marker (IL-10) in the supernatants of macrophages. N = 5. n.s.P > 0.05, ***P < 0.001. NC, negative control; shRNA, short hairpin RNA; VDR, vitamin D receptor; mRNA, messenger RNA; IL, interleukin; TNF, tumor necrosis factor; RT-qPCR, real-time quantitative polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; Exos-shNC, exosomes derived from HaCaT cells with stably transfected shNC; Exos-shVDR, exosomes derived from HaCaT cells with stably transfected shVDR.