FIG. 7.

USF1 and USF2a transactivate the LMP1 promoter independently of EBNA2. The pSG5(USF1) and pSG5(USF2a) expression vectors, separately or mixed, or the pSG5 control vector was cotransfected with the reporter plasmid pgLRS(−106)CAT or pgLRS(−106)(mut−59/−53)CAT or the pgCAT control plasmid into DG75 cells, as detailed in Materials and Methods. The CAT activity is given as percent chloramphenicol acetylation. The values shown are the means from three independent transfections. Error bars indicate standard errors of the means.