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. 2023 Jul 9;13(12):3988–4003. doi: 10.7150/thno.84650

Figure 3.

Figure 3

Gut microbiota-derived OMVs cross the impaired gut-vascular barrier and accumulate in the tubulointerstitium of DM rats. (A-D) The presence of ompF+ OMVs (red dots) in renal tubular marked with AQP-1 (green) was detected by immunofluorescence staining and quantified (n=5). Nuclei were stained with DAPI (blue) (scale bar, 200 μm and 50 μm, original magnification × 400). (E-F) Expressions of tight junction proteins occludin (red) and ZO-1 (green) in colon tissues were detected by immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar, 200 μm, original magnification × 400). **P < 0.01.