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. 2023 Jul 9;13(12):3988–4003. doi: 10.7150/thno.84650

Figure 7.

Figure 7

DM-fBEVs mediate tubulointerstitial inflammation by activating the caspase-11/caspase-1 pathway in vivo. (A) Expression levels of caspase-11 and caspase-1 in tubulointerstitium of rats were determined by immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar, 200 μm, original magnification × 400). (B-D) Protein expression levels of pro caspase-11, active caspase-11, pro caspase-1 and active caspase-1 in the renal cortex of rats were detected by Western blot analysis (n=4-5). (E) Expression levels of caspase-11 and caspase-1 in tubulointerstitium of rats were determined by immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar, 200 μm, original magnification × 400). (F-H) Protein expression levels of pro caspase-11, active caspase-11, pro caspase-1 and active caspase-1 in the renal cortex of rats were detected by Western blot analysis (n=4-5). (I) Expression levels of caspase-11 and caspase-1 in tubulointerstitium of mice were determined by immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar, 200 μm, original magnification × 400). (J-L) Protein expression levels of pro caspase-11, active caspase-11, pro caspase-1 and active caspase-1 in the renal cortex of mice were detected by Western blot analysis (n=5). *P < 0.05; **P < 0.01; ***P < 0.001.