Figure 4.
EVs derived from hypoxic microglia augment AQP4 polarity and attenuate astrogliosis in different regions of the periinfarct cortex and striatum. (A) Schematic diagram of the cortex and striatum at post-MCAO day 7 of interest along with representative immunofluorescence maps. (B-G) Statistical plots of the polarity of AQP4 and GFAP mean fluorescence intensity in the periinfarct region 1 (R1), R2, R3, R4, R5, and striatum (n = 6). The EV-treated mice exhibited a higher polarity of AQP4 in all periinfarct regions and striatum than those of the MCAO control mice. Likewise, the EV-treated mice were associated with a lower GFAP mean fluorescence intensity in the peri-infarct R2, R3, R4, and striatum. *p < 0.05; **p < 0.01; ****p < 0.0001; NS, not statistically significant; MCAO, middle cerebral artery occlusion; EVs, extracellular vesicles; AQP4, aquaporin 4.