FIG. 3.
Southern blot analysis of mutant viruses. Southern blot analysis was performed with DNAs of wild-type PrV-Ka (lanes 1), PrV-ΔgLβ (lanes 2), and PrV-ΔgLPass (lanes 3) after cleavage with BamHI. The upper part of the figure shows a genomic and BamHI restriction map of the PrV genome, with part of the unique short region (US) enlarged and genes of interest indicated by arrows. Locations of hybridization probes B to F are shown by boxes. In the lower part of the figure, the ethidium bromide stained gel is shown in panel A. The other panels depict hybridization with BamHI-fragment 6 (B), a gL-specific probe (C), a gH-specific probe (D), BamHI fragment 7 (E), or a gC-specific probe (G).