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Western blot analysis. Purified virions of PrV-Ka (lanes 1), PrV-ΔgLβ (lanes 2), and PrV-ΔgLPass (lanes 3) were lysed, and proteins were separated in a sodium dodecyl sulfate–10% polyacrylamide gel. After electrophoretic transfer, nitrocellulose membranes were probed with antibodies against gB (A), gC (B), gE (C), gL (D), gH (E), and gD (F). After incubation with peroxidase-conjugated secondary antibody, bound antibody was visualized by chemiluminescence recorded on X-ray films.
