FIG. 6.

Genomic arrangement in PrV-ΔgLPass. (A) BamHI restriction fragment map below a schematic diagram of the wild-type PrV genome. U_L, unique long region; U_S, unique short region; IR, internal repeat; TR, terminal repeat. In the BamHI restriction fragment map, deletions in the gC and gL genes within PrV-ΔgLPass are indicated. (B) Enlargement of the gH gene region and the U_S region. Vertical arrows point to sites of recombination. (C) Arrangement of the U_S region in PrV-ΔgLPass, containing the translocation of part of the gH gene region fused to a duplicated copy of the gD gene. [BamHI] indicates absence of BamHI cleavage site separating fragments 7 and 12, and [ΔgG] denotes the presence of a second, truncated copy of the gG gene. The region verified by sequencing is indicated by a dashed line bounded by arrowheads. (D) Location of the BspEI fragment used for rescue of infectivity in PrV-ΔgLβ. (E) Location of the NdeI fragment contained in cell line Nde-67.