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. 2023 Jul 26;19(7):e1011217. doi: 10.1371/journal.ppat.1011217

Fig 7. LASVpp membrane permeabilization is inhibited by fusion attenuating GPc mutations.

Fig 7

(A) Wild-type and mutant LASVpp fusion with A549 cells measured by a BlaM assay. LASVpp was bound to A549 cells in the cold, and the fusion was initiated by shifting to 37°C and incubating for 2 hours. Data shown are means ± SD of 3 independent experiments. Results were analyzed by Student’s t-test. *, p<0.05; **, p<0.01; ***, p<0.001. (B, C) Mean YFP-Vpr intensity decay of LASVpp mutants on A549 cell surface after applying membrane-impermeable pH 5.0 citrate buffer. The exponential decay rates k are shown in 1/sec. Results were analyzed by Student’s t-test, **, p<0.01; ***, p<0.001; NS, not significant. (D) Quantification of different types of YFP-Vpr quenching of single LASVpp GPc mutants on A549 cells after applying low pH. Data was analyzed by Fisher’s exact test. **, p<0.01; ***, p<0.001; NS, not significant.