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. 2023 Aug 7;12:RP87521. doi: 10.7554/eLife.87521

Table 2. Primer sequences used in the article.

Oligo name Sequence (5' -> 3') Anneal temperature (°C) Application
1 dmist_vir_fw CACAGGGATGTGATGCCGGTTAAC 55 dmistvir genotyping
2 dmist_vir_rev GTAGACACATACTGCCATACCAATC 55 dmistvir genotyping
3 vir_fw CACCAGCTGAAGCCTATAGAGTACGAGC- 55 dmistvir genotyping
4 dmist_Dr_5RACE_fw CGTTTCGCCACAATGTCAGCA 55–65 dmist_Dr 5'RACE
5 dmist_Dr_5RACE_rev_outer AATGTTCAACTCCAGGCGTC 55–65 dmist_Dr 5'RACE
6 dmist_Dr_5RACE_rev_inner AATGTTCAACTCCAGGCGTC 55–65 dmist_Dr 5'RACE
7 dmist_Dr_3RACE_fw_inner GACGCCTGGAGTTGAACATT 55–65 dmist_Dr 3'RACE
8 dmist_Dr_3RACE_fw_outer GGTATGGCAGTATGTGTCTACA 55–65 dmist_Dr 3'RACE
9 Dmist_Mm_3RACE_outer GCTGGTGACTGTCCTCCTTATG 55–65 dmist_Mm 3'RACE
10 Dmist_Mm_3RACE_inner GTGTCTACAAGCCCATCCGTC 55–65 dmist_Mm 3'RACE
11 dmist_Dr_fw TTTCGCCACAATGTCAGCAGC 56 dmist_Dr probe
12 dmist_Dr_rev CGACTTTCATTTATTAGTTCAGACATGTC 56 dmist_Dr probe
13 qPCR_dmist_fw ACGCCAGACCTTATGAAATCC 60 RT-qPCR
14 qPCR_dmist_rev TGCGTCGGAGAGGTTTGTAG 60 RT-qPCR
15 qPCR_ankrd13a_fw TGGTGGCGTTCCAGAGTTAC 60 RT-qPCR
16 qPCR_ankrd13a_rev GGACACGAGAGGAATCCAGC 60 RT-qPCR
17 qPCR_slc6a4b_fw ACATGGTTGGGTCGACGTTT 60 RT-qPCR
18 qPCR_slc6a4b_rev TCCAACCCACCAAAAGTGCT 60 RT-qPCR
19 ef1alpha_fw TGCTGTGCGTGACATGAGGCAG 60 RT-qPCR
20 ef1alpha_rev CCGCAACCTTTGGAACGGTGT 60 RT-qPCR
21 SP6dmist_sgRNA ATTTAGGTGACACTATAGCGTTATGCAGAAAGCGGTGGTTTTAGAGCTAGAAATAGCAAG n/a CRISPR
22 T7atp1a3a_sgRNA TAATACGACTCACTATAGACTGACGGCTCCAAAATGGGTTTTAGAGCTAGAAATAGCAAG n/a CRISPR
23 SP6fxyd1_sgRNA ATTTAGGTGACACTATAGGACCCTCTGCCAACACAAGGTTTTAGAGCTAGAAATAGCAAG n/a CRISPR
24 SP6atp1a3b_sgRNA ATTTAGGTGACACTATAGGACTGACTGCGCAACCATGGTTTTAGAGCTAGAAATAGCAAG n/a CRISPR
25 HRM_dmist_fw GCCACAATGTCAGCAGCACG 59 HRM
26 HRM_dmist_rev GCGTTCACTTTAGACTCTCCCAGC 59 HRM
27 HRM_atp1a3a_fw TGACAGACTGAAGAAACAGC 55 HRM
28 HRM_atp1a3a_rev TTAAATCTCAGCACCAGCAG5 55 HRM
29 HRM_fxyd1_fw TGACCAAACCTTCTTAAGGTGC 58 HRM
30 HRM_fxyd1_rev AAATTGAGAAGACTTACTGGTCTGC 58 HRM
31 HRM_atp1a3b_fw AAAGGCTGTCACTTTCTCCATCAC5 58 HRM
32 HRM_atp1a3b_rev TGCAGTAGATGAGGAATCGGTC 58 HRM
33 MiSeq_dmist_fw TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTATAACTTACGTGTGGACGGACTC 58 MiSeq
34 MiSeq_dmist_rev GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTGCCTCAGCAGGATTTCATAAG 58 MiSeq
35 MiSeq_atp1a3a_fw TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCGTTATCCGTGCAAGAGCTTC 58 MiSeq
36 MiSeq_atp1a3a_rev GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTCTCAGCACCAGCAGTTATCG 58 MiSeq
37 MiSeq_atp1a3b_fw MiSeq_atp1a3b_rev TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGACTGACATTCTCTCTTCGTG 68 MiSeq
38 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTCTCTGTGATGCAGTAGATGAGG 68 MiSeq
39 MiSeq_fxyd1_fw TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAAATACTGTCTTGTGACCAAACC 57 MiSeq
40 MiSeq_fxyd1_rev GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTCATCCTCTGCTGCAAAATGC 57 MiSeq
41 attB1-dreammist forward primer GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGTCAGCAGCACGCCTGATCTCC 55–60 Gateway
42 attB3-dreammist reverse primer GGGGACCACTTTGTACAAGAAAGCTGGGTATCACCTGCGTCGGAGAGGTTTGTAG 55–60 Gateway
43 Dmist-GFPA22WFw GCTTTTCCAGTCTGGGAGTTGGCAGCTGGGAGAGTCTAAAG 66 SDM
44 Dmist-GFPA22WRev CTTTAGACTCTCCCAGCTGCCAACTCCCAGACTGGAAAAGC 66 SDM