| Cell line (human) |
HEK293 |
ATCC |
CRL-1573 |
Routinely subjected to extensive quality control and physiological and molecular characterization (STR profiling). Mycoplasma negativity is regularly confirmed. |
| Antibody |
Anti-CDKL5 (sheep polyclonal) |
University of Dundee |
Anti-CDKL5 350-650 |
1:2000 |
| Antibody |
Anti-pS222 EB2 (rabbit polyclonal) |
Covalab, from Baltussen et al., 2018
|
|
1:1000-1:2000 |
| Antibody |
Anti-EB2 (rat polyclonal) |
Abcam |
ab45767 |
1:1000 |
| Antibody |
Anti-FLAG (rabbit polyclonal) |
Cell Signaling |
14793 |
1:1000 |
| Antibody |
Anti-rabbit IgG HRP (goat polyclonal) |
Jackson ImmunoResearch |
111-035-144 |
1:5000 |
| Antibody |
Anti-tubulin (mouse monoclonal) |
Sigma |
T9026 |
1:100,000 |
| Antibody |
Anti-CDKL5 (rabbit polyclonal) |
Atlas |
HPA002847 |
1:1000 |
| Antibody |
Anti-β-catenin (rabbit polyclonal) |
Cell Signaling |
9562 |
1:1000 |
| Antibody |
Anti-phospho-β-catenin (Ser33/37 Thr41) (rabbit polyclonal) |
Cell Signaling |
9561 |
1:250 |
| Commercial assay or kit |
CDKL5 TE Assay |
Promega |
NLuc-CDKL5 (NV2911); Transfection Carrier DNA; NanoBRET TE Intracellular Kinase Assay, K-11 (N2650) |
|
| Commercial assay or kit |
GSK3α TE Assay |
Promega |
NLuc-GSK3A (NV3191); Transfection Carrier DNA; NanoBRET TE Intracellular Kinase Assay, K-8 (N2620) |
|
| Commercial assay or kit |
GSK3β TE Assay |
Promega |
NLuc-GSK3B (NV3201); Transfection Carrier DNA; NanoBRET TE Intracellular Kinase Assay, K-8 (N2620) |
|
| Commercial assay or kit |
CDK9/Cyclin K TE Assay |
Promega |
NLuc-CDK9 (NV2871); CCNK Expression Vector (NV2661); NanoBRET TE Intracellular Kinase Assay, K-8 (N2620) |
|
| Commercial assay or kit |
CDK16/Cyclin Y TE Assay |
Promega |
NLuc-CDKL16 (NV2741); CCNY Expression Vector (NV2691); NanoBRET TE Intracellular Kinase Assay, K-12 (NF1001) |
|
| Commercial assay or kit |
CDK17/Cyclin Y TE Assay |
Promega |
NLuc-CDKL17 (NV2751); CCNY Expression Vector (NV2691); NanoBRET TE Intracellular Kinase Assay, K-12 (NF1001) |
|
| Commercial assay or kit |
CDK18/Cyclin Y TE Assay |
Promega |
NLuc-CDKL18 (NV2761); CCNY Expression Vector (NV2691); NanoBRET TE Intracellular Kinase Assay, K-12 (NF1001) |
|
| Commercial assay or kit |
scanMAX |
Eurofins DiscoverX Corporation |
|
|
| Commercial assay or kit |
KinaseSeeker
|
Luceome Biotechnologies |
|
CDKL5 only |
| Commercial assay or kit |
Radiometric kinase assays |
Eurofins DiscoverX Corporation |
|
|
| Chemical compound, drug |
B1 (CAF-382) |
This paper, Figures 1—5,7
|
|
Available from senior authors (alison.axtman@unc.edu) |
| Chemical compound, drug |
B4 (HW2-013) |
This paper, Figures 1—3, Figures 7 and 14
|
|
Available from senior authors (alison.axtman@unc.edu) |
| Chemical compound, drug |
B12 (LY-213) |
This paper, Figures 1—3, Figures 7 and 14
|
|
Available from senior authors (alison.axtman@unc.edu) |
