Figure 5.

Histologic Examination of Fibrosis Levels

(A) RV sampling from the SCD heart in relation to optical mapping results (RVOT 1 and RVOT 2 were collected in RVOT biphasic upstrokes islet and RVFW 1 and RVFW 2 in areas without conduction abnormalities; dashed squares). (B) Masson’s trichrome stained transmural sections (6 μm) from a) SCD heart and b) 3 control hearts (for control hearts, 1 sample from each RVOT and RVFW) and associated quantification of fibrosis as percentage of myocardium (each section divided in 8 areas, so n = 8 for each area of each individual; mean ± SEM; ns indicates a nonsignificant difference; fixed P = 0.05; Kruskall-Wallis test followed by the Dunn’s posthoc test). Abbreviations as in Figure 2.