Table 1.
RNA sequencing data from transfer culture for genes with homology to 3,4-DHB pathway genes
| Enzyme | Predicted gene | Mean fructose TPM | Mean 3,4-DHB TPM | Fold change (3,4-DHB vs. Fructose) |
|---|---|---|---|---|
| Protocatechuate 3,4-dioxygenease | NRRL3_01405 | 8.24 | 18,330.48 | 2223.40 |
| NRRL3_02644 | 16.77 | 464.59 | 27.70 | |
| NRRL3_04277 | 1.62 | 0.72 | 0.45 | |
| NRRL3_04787 | 11.46 | 100.19 | 8.74 | |
| NRRL3_05330 | 2.64 | 9.31 | 3.53 | |
| Carboxy-cis,cis-muconate cyclase | NRRL3_02586 | 16.88 | 1016.83 | 60.23 |
| 3-carboxymuconolactone hydrolase/decarboxylase | NRRL3_00837 | 21.48 | 1038.39 | 48.34 |
| NRRL3_01409 | 10.51 | 727.89 | 69.23 | |
| NRRL3_03759 | 1.51 | 6.76 | 4.49 | |
| NRRL3_08340 | 15.20 | 3661.57 | 240.94 | |
| β-ketoadipate:succinyl-CoA transferase | NRRL3_01593 | 32.52 | 55.19 | 1.70 |
| NRRL3_01886 | 20.66 | 1499.22 | 72.58 | |
| NRRL3_11640 | 32.14 | 360.56 | 11.22 | |
| β-ketoadipyl-CoA thiolase | NRRL3_01526 | 19.21 | 1410.97 | 73.47 |
| NRRL3_07786 | 274.13 | 254.02 | 0.93 | |
| NRRL3_11162 | 37.38 | 143.40 | 3.84 |