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Effects of polymerase deficiency on the bypass efficiency and fidelity of GA-FAPy-dfG. TLS efficiency (A) and mutation frequency (B) of GA-FAPy-dfG in a series of isogenic TLS polymerase KO cells. C, mutation spectrum at the site of GA-FAPy-dfG. Assays were performed more than thrice, independently. GA-FAPy-dfG, N6-(2-deoxy-2-fluoro-d-arabinofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-[N-(2-carbamoyl-2-hydroxyethyl)formamido]pyrimidine.
