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Binding parameters for sugar–VhChiP interactions by isothermal titration calorimetry
| Chitohexaose titration |
|||||
|---|---|---|---|---|---|
| WT | Truncated VhChiP | Truncated VhChiP + N-plug peptide |
N-plug peptide | D1A mutant | |
| Substrate (mM) | 0.6 | 5.0 | 1.0 | 30 | 0.8 |
| Protein (μM) | 20 | 20 | 20 | - | 20 |
| N-plug (μM) | - | - | 5 | 500 | - |
| n (site) | 3.2 ± 0.1 | 2.8 ± 0.1 | 2.3 ± 0.2 | 1.0 ± 0.02 | 3.0 ± 0.1 |
| Kd (μM) | 0.3 ± 0.06 | 10.0 ± 0.3 | 2.9 ± 0.07 | 2600 ± 120 | 1.2 ± 0.2 |
