Fig. 5.

Knockdown of Kdm6b upregulates the Wnt signaling pathway. a–j In situ staining of the Wnt signaling target genes, axin2, lef1, tcf7l2, ctnnb1, and ctnnb2 are all upregulated in kdm6bb-MO embryos compared to the controls by in situ hybridization at 32 hpf. k The ratio of axin2, lef1, tcf7l2, ctnnb1, and ctnnb2-positive cells to total cells in the primordia of controls (ConMO; n = 10 embryos for axin2 and lef1, respectively; n = 12, 15, and 15 embryos for tcf7l2, ctnnb1, and ctnnb2, respectively) and experiment group (kdm6bb-MO; n = 12 embryos for axin2, n = 8 embryos for lef1, n = 15 embryos for tcf7l2, n = 12 embryos for ctnnb1, and n = 14 embryos for ctnnb2).The black dotted lines are drawn to shape the primordium. Data are recorded as mean (minimum and maximum values). ****p < 0.0001