Table 1.
Comparison of precursors and their contribution to the cost of eCell expression for PpiB expression using 1.5 g eCells in 25 mL CFPS buffer1
| Precursor/Energy source | Precursor cost (USD) | Labelling level (%) actual/max. | Protein yield (mg) | Cost per reaction | Position labelled in Phe and Tyr |
|---|---|---|---|---|---|
| 3-13C pyruvate | $866/g | 36/50 | 4.8 | $36 | δ |
| 3-13C pyruvate + 1-13C glucose | $866/g + $282/g | 50/100 | 2.7 | $26 | δ |
| 3-13C pyruvate + 4-13C erythrose | $866/g + $1340/g | 70/100 | 1.6 | $104 | δ |
| 2-13C glucose | $305/g | 46/50 | 4.8 | $15 | ε |
1 Prices from Cambridge Isotope Laboratories (https://www.isotope.com) and Omicron Biochemicals, Inc. (https://www.omicronbio.com), Accessed 4 Jan 2023