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. 2023 Jul 21;30(8):2005–2016. doi: 10.1038/s41418-023-01194-1

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 1 — A BMDMs were generated by cultivating bone marrow cells isolated from the femurs of 6–8 weeks old WT and TRAF3^MKO mice. Total RNA was extracted and RNA-sequencing analysis was performed using Illumina Hiseq2500/X platform. Cell proteome expression profile was analyzed using LC-MS/MS analysis. B Volcano map of RNA-sequencing different expression genes. M1/M2 macrophage marker genes are provided. Fold change >1.5, FDR < 0.05, n = 3 for each group. C Volcano map of proteome expression profile. M1/M2 macrophage markers are provided. Fold change >1.5, p < 0.05, n = 3 for each group. D Heat map of M1/M2 macrophage marker gene mRNA and protein levels in WT and TRAF3^MKO BMDMs. E BMDMs were harvested and lysed with RIPA buffer. Immunoblotting assays were performed to analyze M1 and M2 marker expression.