FIG. 2.

Protease sensitivity of SU in the absence and presence of sTva. Soluble EnvA PI was incubated with (+) or without (−) excess sTva and subjected to thermolysin digestion before analysis by SDS-PAGE. The western blot was probed with antiserum against SU, which was detected by ECL. The SU subunit and proteolytic product, SU^therm, migrated as indicated on the left, and molecular weight markers are on the right. The uncleaved form of EnvA PI (Clv⁻ PI) was included as a size reference. wt, wild-type.