FIG. 1.

Structure of cDNA templates for chimeric YF/JE viruses (truncated within the NS1 protein for clarity). YF/JE-S and YF/JE-N refer to the two chimeric viruses as described in the text. The 5′ nontranslated region is derived from YF5.2iv (6). Hatched regions are from JE SA₁₄-14-2, and solid regions are from JE-N. The remainder of the nonstructural region is derived from YF5.2iv. The amount of virus recovered is indicated as the titer of virus (log₁₀ PFU per milliliter) in the media at the time of harvest at 96 h after RNA transfection of Vero cells.