FIG. 3.

Immunoprecipitation of YF and JE proteins with monoclonal and polyclonal antisera. LLC-MK₂ cells were infected and labelled as described for Fig. 2A. Viral proteins were immunoprecipitated from the media with monoclonal antibody to JE (A) or polyclonal antisera to the YF and JE viruses (B and C), as described in Materials and Methods. In panel A, YF, YF/JE-N, and YF/JE-S refer to media from cells infected with these respective viruses. In panel B, YF-infected cells were used. In panel C, YF/JE-N-infected cells were used. NI refers to nonimmune ascites fluid. YF-HI and JE-HI refer to hyperimmune ascites fluid to the YF and JE viruses, respectively. Proteins were analyzed on 10% SDS gels.