Table 3.
Studies reporting Diagnostic Test Accuracy data for cfDNA in AMI.
| Author | Year | Number | Outcome to discriminate |
cfDNA |
Troponin |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| cfDNA quantification method | AUC (95%CI), p-value | Cut-off, units | Sensitivity | Specificity | AUC (95%CI) | Cut-off | Sensitivity | Specificity | ||||
| Agiannitopoulos | 2020 | 80 AMI 50 controls |
AMI | Qubit® 3.0 ssDNA assay | 0.99 (0.997–1.00), p < 0.001 | 20.45 ng/μl | 100% | 99% | 0.94 (0.87–1.00), p < 0.001* | NR | NR | NR |
| Qubit® 3.0 dsDNA assay | 0.94 (0.89–0.98), p < 0.001 | 3.43 ng/μl | 100% | 98% | ||||||||
| NanoDrop® | 0.99 (0.99–1.00), p < 0.001 | 15.4 ng/μl | 100% | 92% | ||||||||
| qPCR TaqMan Copy Number Reference Assay TERT | 0.99 (0.99–1.00), p < 0.001 | 13.70 ng/μl | 100% | 99% | ||||||||
| Cui | 2013 | 49 AMI 60 controls |
ACS | branched DNA-based Alu assay | 0.98 (0.96–1.00) | NR | 92% | 98% | 0.74 (0.68–0.80)** | NR | 57% | 98% |
| STEMI from ACS | branched DNA-based Alu assay | 0.90 (0.84–0.95) | NR | NR | NR | 0.76 (0.67–0.84)** | NR | NR | NR | |||
| Lou | 2015 | 120 AMI 60 controls |
AMI | Alu-based qPCR assay (Primer size 201 bp) | 0.89 (0.82–0.95), p < 0.001 | 3.71 log copies/ml | 77% | 96% | 0.90 (0.85–0.96), p < 0.001** | 1.35 ng/ml | 80% | 84% |
| Alu-based qPCR assay (Primer size 170 bp) | 0.76 (0.66–0.85), p < 0.001 | 1.93 log copies/ml | 100% | 49% | ||||||||
| Alu-based qPCR assay (Primer size 147 bp) | 0.86 (0.79–0.93), p < 0.001 | 0.22 log copies/ml | 100% | 60% | ||||||||
| Alu-based qPCR assay (Primer size 113 bp) | 0.94 (0.89–0.99), p < 0.001 | 3.73 log copies/ml | 87% | 96% | ||||||||
| Alu-based qPCR assay (Primer size 76 bp) | 0.97 (0.93–1.00), p < 0.001 | 6.13 log copies/ml | 87% | 100% | ||||||||
| Alu = log (Alu1 + Alu2 + Alu3 + Alu4 + Alu 5) | 0.93 (0.87–0.99), p < 0.001 | 6.40 log copies/ml | 92% | 96% | ||||||||
| Ren | 2022 | 116 AMI 25 controls |
AMI | methylated CORO6 ddPCR assay | 0.68 (0.59–0.78), p = 0.0037 | NR | NR | NR | NR | NR | NR | NR |
| Xie | 2018 | 100 AMI 30 controls |
AMI | PicoGreen® dsDNA assay | 0.96 (0.90–0.99) | 110 ng | NR | NR | NR | NR | NR | NR |
| Zemmour | 2018 | 31 AMI 83 controls |
STEMI | demethylated FAM101A sequencing-based assay | 0.94 (0.90–0.98), p < 0.001 | NR | NR | NR | NR | NR | NR | NR |
*Cardiac troponin T, **Cardiac Troponin I, NR: Not Reported.