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. 2023 Jul 29;4(3):102458. doi: 10.1016/j.xpro.2023.102458

Assay assembly

Reaction tube # 1 2 3 4
Resuspended microsomes 12 μL 12 μL 12 μL 12 μL
PNGase F (1:4 diluted) 1 μL 1 μL 1 μL
10% NP-40 1 μL 1 μL
10% SDS 1 μL
Buffer T 8 μL 7 μL 6 μL 5 μL
Total = 20 μL