FIG. 6.

Effects of CHX on RANTES mRNA induction by MV in U373 cells. U373 cells (10⁷/25 ml of 10% DMEM/75-cm² flask) were treated with CHX at 100 μg/ml for 30 min and were then infected with MV at an MOI of 10 in the presence of CHX for the times noted. The dose of CHX was predetermined by assessing the maximum level of protein synthesis inhibition by CHX in U373 cells in the absence of cell death, as described in Results. RANTES and MV nucleocapsid mRNAs were examined by Northern blotting (20 μg of RNA/lane). Cyclophilin was used as a control. Results are representative of findings from four separate experiments. The mRNA bands on the autoradiogram were quantitated, and the ratio of the density of RANTES or MV nucleocapsid mRNA to that of cyclophilin mRNA is shown as a histogram.