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Negative stain EM reveals low-resolution differences between some mutants. Representative negative stain images of 2 μM enzyme with either 1 mM ATP and 1 mM MgCl2 (A) or 5 mM GTP (B). The oligomeric and conformational states of some mutants vary from WT. S160del does not form filaments in either condition. G113E and G113R form compressed filaments in the absence of GTP.
