Table 1.
E12.5 TEC preferentially adopt a cortical TEC fate.
| Location and number of mGFP+ foci in recovered graft | |||
|---|---|---|---|
| Graft ID | Cortex | Medulla | CMJ |
| 60 E12.5 PLET1+mGFP+ cells injected into each E12.5 lobe | |||
| G1-60 | 40-45 | 0 | 0 |
| G2-60 | 10-15 | 6 | 2 |
| G3-60 | 15-20 | 0 | 3 |
| G4-60 | 5-10 | 2 | 0 |
| G5-60 | 30-35 | 0 | 0 |
| G6-60 | 15-20 | 0 | 0 |
| G7-60 | 5-10 | 0 | 0 |
| G8-60 | 5-10 | 0 | 0 |
| 20-30 E12.5 PLET1+mGFP+ cells injected into each E12.5 lobe | |||
| G1-20 | 0-5 | 4 | 2 |
| G2-20 | 10-15 | 0 | 0 |
| G3-20 | 5-10 | 0 | 0 |
| G4-20 | 10-15 | 6 | 2 |
| G5-20 | 5-10 | 0 | 0 |
| G6-20 | 5-10 | 0 | 0 |
| G7-20 | 5-10 | 0 | 0 |
| G8-20 | 5-10 | 0 | 0 |
| 10 E12.5 PLET1+mGFP+ cells injected into each E12.5 lobe | |||
| G1-10 | 6 | 0 | 0 |
| G2-10 | 4 | 0 | 0 |
| 5 E12.5 PLET1+ mGFP+ cells injected into each E12.5 lobe | |||
| G1-5 | 1-5 | 0 | 0 |
| 1 E12.5 PLET1+ mGFP+ cell injected into each E12.5 lobe | |||
| G1-1 to G83-1 | 40-45 | 0 | 0 |
Table shows distribution of foci in grafted E12.5 thymic lobes that had been injected with 1, 5, 10, 20-30 or 60 E12.5 mGFP+PLET1+ TEC. Grafted were placed under the kidney capsule on the same day and recovered for analysis two-three weeks later. mGFP+ foci were scored visually for regional localization based on the DAPI, α-K14, Ly51 and CDR1 staining. Some grafts contained mGFP+ TEC in both the cortical and medullary compartments but most had mGFP+ cells in the cortex only. No grafts were scored with contribution to the medulla only. 60 cells injected, n= 8 independent grafts of which 8 grafts contained mGFP+ cells (n=8/8); 20-30 cells injected, n= 22 independent grafts of which 8 grafts contained mGFP+ cells (n=8/22); 10 cells injected, n= 8 independent grafts of which 2 grafts contained mGFP+ cells (n=2/8); 5 cells injected, n= 18 independent grafts of which 1 graft contained mGFP+ cells (n=1/18); 1 cell injected, n= 83 independent grafts of which 1 graft contained mGFP+ cells (n=1/83). N for each condition represents an independent graft; at least three biologically independent replicates were performed for each injection condition. Only grafts that contained mGFP+ cells upon visual inspection were analyzed by immunohistochemistry.