Table 2.
Cell identity not community effect determines progeny location.
| Location and number of mGFP+ foci in recovered graft | ||||
|---|---|---|---|---|
| Graft ID | Cortex | Medulla | CMJ | Other |
| 40 E12.5 PLET1+mGFP+ cells injected into the lumen of each E11.5 lobe | ||||
| G1-L | 5-10 | 0 | 0 | 0 |
| G2-L | 5-10 | 2 | 0 | 0 |
| G3-L | 10-15 | 0 | 0 | 0 |
| 30 E13.5 UEA1+mGFP+ cells injected into each E12.5 lobe | ||||
| G1-UEA1 | 0 | 3 | 0 | 1* |
| G2-UEA1 | 0 | 1 | 0 | 4* |
| G3-UEA1 | 0 | 2 | 1 | 0 |
| 30 E11.5 PLET1+mGFP+ cells injected into each E12.5 lobe | ||||
| G1- E11.5 | 10-15 | 0 | 0 | 0 |
*Cells classified as ‘other’ were not cTEC but were not present in clear medullary areas. They were Ly51-UEA1+K14-and were present either as cyst-like structures or as cells with uncharacteristically large vacuoles.
Table shows distribution of foci in grafted E12.5 or E11.5 thymic lobes that had been injected with 20 or 40 mGFP+PLET1+ TEC of the developmental age shown. The injected cells were placed in either the body or the lumen of the lobe, as indicated. Grafted were placed under the kidney capsule on the same day and recovered for analysis two-three weeks later. mGFP+ foci were scored visually for regional localization based on the DAPI, α-K14, Ly51 and CDR1 staining. 40 E12.5 mGFP+PLET1+ TEC into E11.5 lobe lumen, n≥5 independent grafts of which 3 grafts contained mGFP+ cells (n=3/≥5); 30 E13.5 mGFP+UEA1+ TEC injected into E12.5 lobe body, n= 40 independent grafts of which 3 grafts contained mGFP+ cells (n=3/40); 30 E11.5 mGFP+PLET1+ TEC injected E12.5 lobe body, n= 4 independent grafts of which 1 graft contained mGFP+ cells (n=1/4). N for each condition represents an independent graft; at least three biologically independent replicates were performed for each injection condition. Only grafts that contained mGFP+ cells upon visual inspection were analyzed by immunohistochemistry.