Table 1.

Binding affinities of Bim BH3 and Bmf BH3 with Bcl-2, Bcl-xL and Mcl-1.

Proteins	Bcl-2	Bcl-xL	Mcl-1
Peptide (Kd)
Bim BH3	6.1 ± 0.3 nM	4.4 ± 0.9 nM	5.8 ± 0.1 nM
Bmf BH3	5.2 ± 0.3 nM	5.1 ± 0.4 nM	185 ± 9.6 nM

Fluorescence polarization assay was performed to determine the dissociation constants (Kd) of fluorescently labeled Bim BH3 or Bmf BH3 bound to Bcl-2, Bcl-xL, and Mcl-1. All assays were independently performed three times, and Kd was determined by nonlinear regression analysis.