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Primer sequence, optimal annealing temperatures (OAT) of genes selected for analysis by real-time PCR
| Name | Sequence(5′–3′) | OAT (°C) | efficiency |
|---|---|---|---|
| igf1-QF | GCCATCCAAATCCTCACGCTC | 59 | 104.5% |
| igf1-QR | CTGTTGCCTGTGTTCCCGC | ||
| β-actin-QF | GACCGAGGCACCCCTGAAC | 54.9 | 102.4% |
| β-actin-QR | GATGGGCACTGTGTGTGTGAC |
