FIG. 5.

Induction of IFN-regulated genes by recombinant DuIFNs in primary duck hepatocytes. Cell cultures were incubated for 16 h with 1/50 dilutions of 72-h culture supernatants from COS cells transfected with the expression constructs 6.1.7 and 3.1.2, respectively, both encoding DuIFN-γ, and with similar supernatants from COS cells transfected with an expression construct encoding DuIFN-α or from mock-transfected COS cells. Samples of total RNA (20 μg) were subjected to Northern blot analysis. The blot was sequentially hybridized with radiolabeled chicken IRF-1 (16), duck Mx (1), chicken GBP (28), and chicken glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (22) cDNA probes.