Figure 2.

Modular microfluidic workflow used in this paper in schematic form (see Supporting Figure S4, SI, for the detailed chip design). Droplets containing CAZyme-expressing cells are generated in a flow-focussing device. The cells are grown in droplets and express the encoded CAZymes, followed by a picoinjection with a lysis solution that also contains the reagents for subsequent coupling reactions. Finally, the droplets are sorted based on the reaction progress (measured by the emergence of an absorbance signal) within a defined incubation period.