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. 2023 Jul 24;26(3):390. doi: 10.3892/ol.2023.13976

Figure 1.

Figure 1.

SENP1 is predicted and confirmed as a direct target of miR-122. (A) Correlation of miR-122 expression with SENP1, SENP2, SUMO1 and SUMO3. (B) RT-qPCR determined the expression of miR-122 in HepG2 cells after transfection with the miR-122 or NC mimic. The potential binding sites of miR-122 on the SENP1 3′ untranslated region were (C) predicted by ENCORI and (D) validated by the dual-luciferase reporter assay. The mRNA and protein expression levels of SENP1 in cells transfected with miR-122 or NC mimic was detected by (E) RT-qPCR and (F) western blotting, respectively. ***P<0.001 vs. the NC mimic. miR, microRNA; SENP1, sentrin-specific protease 1; SENP2, sentrin-specific protease 2; SUMO1, small ubiquitin-like modifier 1; SUMO2, small ubiquitin-like modifier 2; RT-qPCR, reverse transcription-quantitative PCR.