Figure 3.

miR-122/SENP1 axis confers chemoresistance to HepG2 cells. HepG2 cells were transfected with the miR-122 mimic and/or SENP1 overexpression before the following experiments. Untransfected cells were considered as the control. The chemoresistance to (A) DOX and (B) sorafenib was determined by Cell Counting Kit-8 after being exposed to the different concentrations. (C) Cell proliferation and (D) migration abilities were assessed by colony formation and Transwell assays, respectively. Scale bar, 100 µm. (E) The protein expression of SENP1 and multidrug resistance associated proteins P-gp and MRP were detected by western blotting. *P<0.05, **P<0.005 and ***P<0.001 vs. the control; ^#P<0.05 and ^###P<0.001 vs. the miR-122. miR, microRNA; DOX, doxorubicin; P-gp, P-glycoprotein; SENP1, sentrin-specific protease 1; MRP, multidrug resistance protein.