Table II.
Detailed information of the antibodies used in western blotting.
| Antibody | Manufacturer | Cat. no. | Dilution |
|---|---|---|---|
| Sentrin-specific protease 1 | ProteinTech Group, Inc. | 25349-1-AP | 1:2,000 |
| Multidrug resistance protein | Beyotime Institute of Biotechnology | AF7503 | 1:1,000 |
| P-glycoprotein | Beyotime Institute of Biotechnology | AF2245 | 1:1,000 |
| Wnt1 | Beyotime Institute of Biotechnology | AF8349 | 1:2,000 |
| β-catenin | Beyotime Institute of Biotechnology | AC106 | 1:1,000 |
| Flag | Sigma-Aldrich | F2555 | 1:250 |
| Myc | Thermo Fisher Scientific, Inc. | PA1-981 | 1:2,000 |
| GAPDH | Beyotime Institute of Biotechnology | AF1186 | 1:1,000 |
| Goat anti-rabbit IgG H&L HRP conjugate | Thermo Fisher Scientific, Inc. | 31460 | 1:15,000 |
secondary antibody