Figure 10.

In-gel fluorescece labeling using the library of probes and TAMRA-PEG₃-N₃ as a reporter tag. (A) The CETZOLE-1 probe (64) (20 μM) and the warhead probe (65) (10 μM) show almost identical labeling profiles, indicating that the replacement of the cyclopentenyl ring does not alter the protein labeling profile. (B) The intensity of the bands observed with probe (65) by in-gel fluorescence increases in a dose dependent manner. (C) The labeling of almost all identified bands diminishes by preincubation with iodoacetamide (I.A) (0.2 and 2 mM). (D) RSL3 (10 μM), ML162 (10 μM) show overlapping small molecule interactome. As expected no competition was observed with erastin (20 μM). Left: optimum exposure of the competition gel, no band is saturated. Right: long exposure ( ~2 min) of the same gel.