Skip to main content
. Author manuscript; available in PMC: 2023 Aug 8.
Published in final edited form as: J Med Chem. 2022 Aug 19;65(17):11788–11817. doi: 10.1021/acs.jmedchem.2c00909

Figure 8.

Figure 8.

Protein pulldown protocol and chemical structures of compounds used for the chemoproteomic analysis following ABPP approach. (A) Simplified cartoon representation of the ABPP approach followed for chemoproteomic analysis of the heterocyclic warheads. Briefly, NCI-H522 cells were treated with appropriate probes for 1 h. Cells were collected and lysed. Then lysates were subjected to CuAAC with either TAMRA-PEG3-N3 (62) or Biotin-TAMRA-PEG3-N3 (63) under standard click conditions. (B) Chemical structures of the affinity and reporter tags used. (C) Library of the probes designed for the ABPP approach. Analog (64) serves as a probe that is structurally related to the original CETZOLE series. Probe (65) serves as the ferroptosis inducing heterocyclic fragment probe, while probes 66–68 serve as inactive controls.