FIG. 5.

Viral RNA in the CNSs of infected mice as assayed by dot blot hybridization. GDVII- and mutant-infected mice were sacrificed on days 5 and 6 p.i., respectively. Total cytoplasmic RNA was extracted from the brains and spinal cords of infected mice, loaded on the membrane (in the amounts [in micrograms] indicated at the top) and hybridized with ³²CTP-labeled PCR-amplified, virus-specific DNA. The numbers on the left correspond to individual mice.