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. 2023 Aug 8;22:120. doi: 10.1186/s12944-023-01880-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — A Laser confocal scanning microscopy images of FYGL in 3T3-L1 cells at 200 × magnification. 3T3-L1 cells were incubated with FITC–FYGL (200 μg/mL) for 4 h; blue (DAPI labelled), red (rhodamine labelled) and green (FITC labelled) represent the nucleus, cytoskeleton and FYGL, respectively. The scale bar represents 100 μm. B Flow cytometric analysis of fluorescence. C Geometric means calculated by FlowJo software. The data are presented as the mean ± S.D. values (n = 3). ^***P < 0.001 vs. control group. D Effect of FYGL on cell viability. 3T3-L1 cells were incubated with various concentrations of FYGL (0, 50, 100, 200, 400 and 800 μg·mL.⁻¹) for 24 h, and cell viability was determined by a CCK-8 assay. The mean ± S.D. values are presented (n = 6)