Figure 2.

PR-619 inhibited cell viability and induced apoptosis in human chondrosarcoma cells. A. JJ012 and SW1353 cells were treated with 2.5 and 5 μM of PR-619 for 24 and 48 h, respectively, after which they were harvested for MTT assays. B. JJ012 and SW1353 cells were treated with 2.5 and 5 μM PR-619 for 48 h; DMSO treatment was considered the untreated control group. Flow-assisted cell sorting with propidium iodide and annexin V-FITC staining was used to quantify apoptotic cells. C. Cell lysates were harvested after PR-619 treatment for 48 h. Following this, the expressions of cleaved caspase-3, cleaved PARP, and phosphorylated histone H2A.X were analyzed via Western blotting analysis. Results are representative of three independent experiments and data are presented as mean ± SD values; *P<0.05 was considered significant as compared with the control. Full-length blots are presented in Figure S1.