Table 3.
Physicochemical properties of the wild-type calreticulin and the p.E381A variant
| Parameters | Wild type | p.E381A | |
|---|---|---|---|
| Number of amino acids | 417 | ||
| Molecular weight | 48141.56 | 48083.82 | |
| Theoretical pI | 4.29 | 4.3 | |
| Number of negatively charged residues | 109 | 108 | |
| Number of positively charged residues | 50 | 50 | |
| Total number of atoms | 6598 | 6592 | |
| Estimated half-life | (Mammalian reticulocytes, in vitro) | 30 hours | |
| (Yeast, in vivo) | >20 hours | ||
| (Escherichia coli, in vivo) | >10 hours | ||
| Instability index | 47.56 | 46.78 | |
| Aliphatic index | 59.14 | 59.38 | |
| Grand average of hydropathicity | -1.104 | -1.092 | |