Figure 2.

Effect of Stattic treatment on lung compliance, collagen levels, pathological lung fibrosis, and inflammatory cytokine levels. (A) Quasi-static compliance in negative control mice, and in mice injected with zymosan then treated with either Stattic or saline (positive controls). (B) Hydroxyproline levels in negative control mice, and in mice injected with zymosan then treated with either Stattic or saline (positive control). * (C) hematoxylin- and eosin-stained lung sections showed patchy regions of disease and inflammation, Scale bar, 200 μm. (D, E) Immunohistochemical staining showing α-SMA-positive fibroblasts (final magnification, ×400) and semi-quantitative analysis of α-SMA-positive cells, scale bar, 200 μm. (F) Protein levels of α-SMA, Fn, Col1, STAT3, and phosphorylated STAT3 in lung tissue. (G to J) BAL fluid levels of TNF-α, IFN-γ, IL-1β, and IL-6 in mice, as assessed by ELISA.

*p < 0.05.

**p < 0.01.