Figure 2.

PI3K/AKT signaling in IBD patients and Caco-2/RAW264.7 co-culture model. (A) Expression levels of PI3K, AKT, and LncCDKN2B transcripts in CD and UC patients and healthy controls (B) and in the Caco-2/RAW264.7 co-culture model after treatment with 2 µM of A83-01. LPS-treated and LPS-untreated cells in the co-cultured system were considered as the control (Ctrl) and non-inflamed groups. (C) Protein changes of p-AKT and p-RPS6 after treatment with A83-01. β-actin was used as a loading control. (D) The histogram represented the intensity of proteins for each group which was normalized to the β-actin band. Results are presented as the mean ± SEM. In vitro analyses were performed in three biological replicates. *, **, and *** refer to the adjusted P value < 0.05, <0.01, and <0.001, respectively.