Table 1.

Potential regions to design PCR primers for the discrimination of Albizia odoratissima (KX852437), A. bracteata (MN709837) and A. kalkora (MW420923).

No.	Species	Forward region start-end	Inverse region start-end	Location of DNA barcodes	Forward sequences	Inverse sequences
1	A. odoratissima	11122–11177	11227–11276	atpA	AAAACATTAAATGAGATTTTTAATCAATTTTTTTCTACTTGTTCCTGAAGTAAAAA	AAAACATTAAATGAGATTTTTAATCAATTTTTTTCTACTT  GTTCCTGAAGTAAAAA
	A. bracteata	12134–12189	12239–12288
	A. kalkora	12056–12111	12161–12210
2	A. odoratissima	12004–12038	12082–12134	atpA	ACTACAATAGTGTATTCCATTGCTCCCCTTTCTTG	ACTACAATAGTGTATTCCATTGCTCCCCTTTCTTG
	A. bracteata	13016–13050	13094–13146
	A. kalkora	12938–12972	13016–13068
3	A. odoratissima	14953–15088	15141–15197	atpH-atpI	CCGACGGCCAACCCAGCAGCAATAACCGAAGCGGCAGAAATCAGTGGATTCATG  ATAAGTTCCTCGCACCAAAAATAAAGAAATAGTTAATGATAC  AATCAACCAACAAATTATGACTTAATTATTCCATCATTAA	CCGACGGCCAACCCAGCAGCAATAACCGAAGCGGCAGAAATCAGT  GGATTCATGATAAGTTCCTCGCACCAAAAATAAAGAAATAGTTAA  TGATACAATCAACCAACAAATTATGACTTAATTATTCCATCATTAA
	A. bracteata	16000–16135	16186–16242
	A. kalkora	15867–16002	16052–16108
4	A. odoratissima	16903–17138	17203–17260	atpI-rps2	GAACAGCTATAATGGCTGAAACTAATAAAATAGCAATTACAACCCAAGAAGTAA  TAAGTACTTGGGCATGGACTTGGAAACCCCCTATTTGCCAATAGAAATGTTGG  CCTACTTCCACAGCCGATATATCGTATAATCTTTTTAATGTGTTGATGGAACATAAT  AGAACATTCATATTGCCCTCTGAAAGAAATACAACTTTAAAAGGAATTATTTTGA  TTCAACCATCTCTTTTT	GAACAGCTATAATGGCTGAAACTAATAAAATAGCAATTACAACCC  AAGAAGTAATAAGTACTTGGGCATGGACTTGGAAACCCCCTATTT  GCCAATAGAAATGTTGGCCTACTTCCACAGCCGATATATCGTATAATC  TTTTTAATGTGTTGATGGAACATAATAGAACATTCATATTGCCCTCT  GAAAGAAATACAACTTTAAAAGGAATTATTTTGATTCAACCATCTCTTTTT
	A. bracteata	18162–18397	18470–18527
	A. kalkora	17979–18214	18287–18344
5	A. odoratissima	17203–17260	17312–17609	atpI-rps2 and rps2	TTTTTTGCGCGATTCAGTAATAGTATAGTAACCGATTCCATAAATCTATGGAGTTCCC	TTTTTTGCGCGATTCAGTAATAGTATAGTAACCGATTCCATAAATC  TATGGAGTTCCC
	A. bracteata	18470–18527	18585–18882
	A. kalkora	18287–18344	18396–18693
6	A. odoratissima	18053–18078	18123–18225	rps2-rpoC2	GATCAGGTATCCTGAAATAGAAATAA	GATCAGGTATCCTGAAATAGAAATAA
	A. bracteata	19323–19348	19393–19490
	A. kalkora	19135–19160	19205–19302
7	A. odoratissima	29593–29618	29664–29713	rpoB-trnC-GCA	AATACTGATTAGTTATGTCTCAATTT	AATACTGATTAGTTATGTCTCAATTT
	A. bracteata	30855–30880	30926–30975
	A. kalkora	30674–30699	30749–30798
8	A. odoratissima	29664–29713	29782–29830	rpoB-trnC-GCA	ATTGAAGAATCATTCATGAATAGTTAATGGTTCCGATTTGTCCTGAATTT	ATTGAAGAATCATTCATGAATAGTTAATGGTTCCGATTTGTCCTGAATTT
	A. bracteata	30926–30975	31042–31090
	A. kalkora	30749–30798	30866–30914
9	A. odoratissima	52029–52062	52137–52211	intron of trnL-UAA	ATCGAAACTCCAGAAAAGAAAGGATCAAGGATGA	ATCGAAACTCCAGAAAAGAAAGGATCAAGGATGA
	A. bracteata	52899–52932	53003–53077
	A. kalkora	52790–52823	52898–52972
10	A. odoratissima	5103–5133	5205–5326	matK-rps16	AAAAGAAATAAAGAAAGAAAAGAAGTTCTTT	AAAAGAAATAAAGAAAGAAAAGAAGTTCTTT
	A. bracteata	5135–5165	5242–5363
	A. kalkora	5137–5167	5234–5355

Note: The regions were sorted by the K-2p distance of the DNA barcodes between each pair of regions.