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. Author manuscript; available in PMC: 2023 Aug 8.
Published in final edited form as: Sci Immunol. 2023 Mar 31;8(81):eade3525. doi: 10.1126/sciimmunol.ade3525

Figure 4. Mtb-specific T cells can be generated in lymphoid organs and CD8+ γδ T cells primed by non-Mtb antigens can respond to Mtb-whole cell lysate.

Figure 4.

(A) Percentage and number of CD8+ γδ T cells in unstimulated and Mtb-lysate (10μg/ml)-stimulated tonsil organoid cultures (day 7) from three children (T1-T3) with no known exposure to TB. (B) Clonal composition of CD8+ γδ T cells in unstimulated and Mtb-lysate-stimulated tonsil organoid cultures from three donors. The total number of paired γδ TCRs is shown below each pie chart. For each TCR expressed by two or more cells (clonally expanded), the absolute number of cells expressing that TCR sequence is shown by a distinct colored section. (C) Stacked bar plots showing the Vγ/δ chain pairings used by the clonally expanded tonsillar CD8+ γδ T cells in the unstimulated and Mtb-lysate stimulated cultures. (D) CDR3δ/γ sequences from two CD8+ γδ TCRs that showed clonal expansion (appeared two or more times) in both unstimulated and Mtb-stimulated organoid cultures. Jurkat α-β-cells expressing these TCRs were tested for response to Mtb lysate (10, 30, 50 and 100μg/ml) and HMBPP (5μM). P-values were determined using one-way ANOVA followed by Dunnett’s multiple comparisons test. Error bars represent mean and standard deviations.