Table 1.
Comparison of experimental protocols, animals used, and findings between previous studies and the present study
| Iaccarino et al. (2016), Nature 540:230–235 | Soula et al. (2023), Nature Neuroscience 26:570–578 | Present study | |||||
|---|---|---|---|---|---|---|---|
| Alzheimer’s disease model | 5xFAD mice | TauP301S mice | 5xFAD mice | 5xFAD and APP/PS1 mice | 5xFAD mice | ||
| Animal gender | Male | Male | Male and female | Not specified | Male | ||
| Animal age | 3 months old | 6 months old | 4 months old | 4 months old | 7 months old | 7 months old | 1 |
| Visual stimulation protocol | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h | LED light flickering at 40 Hz for 1 h |
| Number of sessions | A single session | Repeat daily for 7 d | Repeat daily for 7 d | A single session | Repeat daily for 7 d | A single session | Repeated daily for 5 weeks |
| Effect on microglial cell body diameter and the area occupied by microglia | Increase in microglial cell body diameter and the area occupied by microglia in the visual cortex | Not determined | Increase in microglial cell body diameter and the area occupied by microglia in the visual cortex | Not determined | Not determined | No difference in microglial cell body diameter and the area occupied by microglia in the visual cortex | No difference in the area occupied by microglia in the visual cortex and hippocampus |
| Effect on Aβ plaque load | Not determined | Decreased Aβ plaque number and size by >60% in the visual cortex | Not determined | No difference in the area occupied by Aβ load in the visual cortex and hippocampus | No difference in the area occupied by Aβ load in the visual cortex and hippocampus | No difference in the area occupied by Aβ load in the visual cortex | No difference in the area occupied by Aβ load in the visual cortex and hippocampus |
| Number of animals tested | n = 4 mice per group × 2 groups | n = 8 mice per group × 2 groups | n = 8 mice per group × 2 groups | n = 20–22 mice per group × 2 groups | n = 11–12 mice per group × 2 groups | n = 3–7 mice per group × 2 groups | n = 4 mice per group × 4 groups |
| Statistical analysis used | Unpaired t test | Unpaired t test | Unpaired t test | Two-sided Wilcoxon test | Two-sided Wilcoxon test | Two-sided Wilcoxon test | One-way ANOVA |
1Seven-month-old at the first visual stimulation session, and eight-month-old after the last session.